Field equipment

in situsampling

SCUBA equipment

  • personal SCUBA equipment, ABC, wet suit, regulator, BC, dive computer, personal dive sonar
  • compressor for tank filling (110 l/min, Bauer Utilus 10B)
  • 3 x 10 l tank, steel
  • 2 x 12 l tank, steel
  • 1 x 4 l tank, steel

field data logger

  • 6 x temperature (U22-001, Onset HOBO)
  • 5 x temperature + light (UA-002-64, Onset HOBO)
  • 4 x temperature + light (MX2202, Onset HOBO)
  • 2 x conductivity/salinity (U24-002-C, Onset HOBO)
  • 2 x pH (MX2501, Onset HOBO)
  • 2 x water level (U20L-01 (0-9 m); U20L-02 (0-31 m), Onset HOBO)
  • 5 x cosine PAR (Odissey)

Hydrography

CTD with fluorescence, turbidity sensors

  • SBE 19plus V2 SeaCAT Profiler CTD (max. 600 m) incl. lowering frame (Seabird)
  • Cyclops-7F™ fluorescence sensor (Turner Designs)
  • Turbidity Meter (Seapoint)

water sample bottles

  • 2 x Niskin water samplers, 2.5 L with messenger (General Oceanics)
  • 1 x Niskin water sampler, 5 L with messenger (Hydrobios)

winch

  • hand winch with 100 m, 2.5 mm stainless steel rope (HYDRO-BIOS)

Plankton sampling

Plankton nets

  • Apstein type plankton net, 20 µm (diam. aperture 0.25 m, length 0.5 m, HYDRO-BIOS)
  • Apstein type plankton nets with cone, 20, 55 µm (diam. aperture 0.17m, length 1 m, HYDRO-BIOS)
  • Apstein type plankton nets, 100, 335, 500, 1000 µm (diam. aperture 0.4 m, length 1 m HYDRO-BIOS)                     

flow meter

  • digital, mechanical flow meter with standard rotor, 2030R (General Oceanics)
  • digital, mechanical flow meter, low velocity, 2030R6 (General Oceanics)

Laboratory equipment

General lab. equipment

Water purification system

  • Elix® Advantage 3 for type 2 pure water (Merck-Millipore)

pH-Meter

  • (PB-11 + PY-P11, Sartorius)

magnetic stirrer

  • 2 x Variomag monotherm

balances

  • 0-2100 g, 0.1 g (GE2101, Sartorius)
  • 0-610 g, 0.01 g (BA 610, Sartorius)
  • 0-210 g, high precision, 0.01 mg (RC 210D, Sartorius)

drying oven

  • 25-300°C (Carbolite)

muffle furnace

  • 25-1100°C (Nabertherm)

general lab fridge incl. freezer compartment (Becken)

general lab freezer, horizontal (Whirpool)

lab glassware dishwasher (Whirpool)

Ultrafreezer, -80°C

filtration equipment

filtration device

  • 2 x filtration devices with 2 x 5 positions for 47 mm diam. inline filter holder (custom build)
  • 2 x filtration devices with 2 x 6 positions for 25 mm diam. inline filter holder (custom build)
  • 4 x plywood stands for compact jerrycans (custom build)
  • 20 x compact jerrycan, 5 l with spigot (Bürkle)
plywood stands with compact 5 L jerrycans and filtration devices for 25 mm inline filter holder

vacuum pumps

  • 2 x vacuum pumps (N035AT.18, KNF)
  • 1x vacuum pump
  • 1x vacuum pump (KNF)

vacuum bottles

  • 4 x 20 L heavy-duty vacuum bottles (Nalgene)

Dissolved nutrients analysis (PO43-, Si(OH)4, NO32-, NO2)

micropipettes

  • monocanal micropipettes: 5-40 µl, 40-200 µl, 200-1000 µl, 1-5 ml, 1-10 ml (Finnpipette)
  • repetitive micropipettes: various volumes acc. to pipette tip volumes (HandyStep, Brand)

peristaltic pump for nitrate to nitrite reduction

  • Dinko D-25V

UV/VIS spectrophotometer

  • UVmini-1240, Shimadzu

Laboratory Fluorometer

  • Trilogy (Turner Designs) with absorbance modules for
    • nitrate,
    • phosphate,
    • silicate

Phytopigment analysis

system for filter homogenization and pigment extraction and analysis

  • Mini-Beadbeater-1 (Biospec Products)
  • centrifuge (MiniSpin, 2 ml tubes, 13400 rpm, Eppendorf)
  • access to: UHPLC system (Nexera 2, Shimadzu)

Laboratory Fluorometer

  • Trilogy (Turner Designs) with fluorescence modules for
    • Chlorophyll in vivo,
    • Chlorophyll Extracted – Acidification,
    • Chlorophyll Extracted – Non-Acidification

Picophytoplankton

Flow cytometer for characterisation and enumeration of prokaryotic and eukaryotic picoplankton                                                                                     

  • CytoFLEX equipped with blue and red laser (Beckman Coulter)
Flow Cytometer, CytoFLEX, Beckman Coulter

Nanoplankton

light microscope for observation of coccolithophores, auto- and heterotrophic nanoflagellates

  • compound microscope with brightfield, DIC, cross-polarized light and epifluorescence with digital imaging system (DM2700 P, Leica)
Compound microscope, DM2700 P, Leica Microsystems

Microphytoplankton

Inverted microscope

  • inverted microscope for cell isolation, identification and enumeration with brightfield and phase contrast, compact digital imaging for documentation (TMS-F, NIKON)
  • inverted microscope for cell isolation, identification and enumeration with brightfield, darkfield, phase contrast, DIC and epifluorescence, digital imaging system for documentation (DMi8. Leica)
Inverted microscope, DMi8, Leica Microsystems

Compound microscope

  • compound microscope for general examination and enumeration with brightfield, compact digital imaging for documentation (AxioStar Plus, ZEISS)

compound counting chambers

  • Plankton sedimentation and counting chamber acc. to Utermöhl (2 x 100 ml, 6 x 50 ml, 2 x 10 ml settling cylinders, 6 x plate settling chamber, HYDRO-BIOS)
  • 6 x counting slide acc. Sedgewick-Rafter

Zooplankton

Sample preparation

  • Folsom plankton splitter (Wildco)

dissecting microscope

  • dissecting microscope with compact digital imaging (Stemi DV4, Zeiss)
  • dissecting microscope (Wild)
  • dissecting microscope (Leica)

cell cultivation

  • PAR scalar irradiance sensor (QSL-2100, Biospherical Instruments)
  • 2 x temperature controlled incubator (143 l vol.) (INCU-Line 150R PREMIUM, VWR®) with controlled LED lighting system
  • 2 x temperature controlled incubator (245 l vol.) (INCU-Line 250R PREMIUM, VWR®) with controlled LED lighting system
incubator with temperature control and LED lighting
250 L incubator with temperature control and LED lighting